Program

10.00-11.00 Registration

11.00-12.45 Theory Part 1: Introduction to genome editing (A. Moglia)

12.45-14-00 Lunch

14.00-15-00 Theory Part 2: Introduction to genome editing (A. Moglia)

15.00-17.00 Theory: In vitro cultures and regeneration of plants (I. Perrone)

17.00-17.30 Coffee Break

17.30-18.30 On-line seminar W. Busch: "Harnessing Plants to Address Climate Change using Genome Editing and Genetic Engineering"

9.00-10.45  From theory to practice: Design of guide RNAs (A. Moglia)

10.45-11.15 Coffee break

11.15-13.00 From theory to practice: Systems for the delivery of CRISPR-Cas

Virus-Induced Genome Editing (A. Moglia)

13.00-14.15 Lunch

14.15-18.15 From theory to practice: Systems for the delivery of CRISPR-Cas

• Rice stable genetic transformation - Part I (V. Brambilla, L. Mineri)

• Use of ribonucleoproteins in protoplasts - Part I (I. Perrone, L. Nerva)

08.30-10.00 From theory to practice: Systems for the delivery of CRISPR-Cas

• Rice stable genetic transformation - Part II (V. Brambilla, L. Mineri) - group 1

• Use of ribonucleoproteins in protoplasts - Part II (I. Perrone, L. Nerva) - group 2

10.00-10.30 Coffee Break

10.30-12.00 From theory to practice: Systems for the delivery of CRISPR-Cas

• Rice stable genetic transformation - Part II (V. Brambilla, L. Mineri) - group 2

• Use of ribonucleoproteins in protoplasts - Part II (I. Perrone, L. Nerva) - group 1

12.00-13.00 Seminar by C. Lorenzo: "Using multiplex genome editing to steer yield improvements in maize"

13.00-14-15 Lunch

14.15-18.00 From theory to practice: Tools for the identification of mutations induced by genome editing

• with Sanger approach (C. Comino)

• with Next Generation Sequencing (A. Acquadro)